|
KMID : 0616120160470040360
|
|
Korean Journal of Pharmacognosy
2016 Volume.47 No. 4 p.360 ~ p.365
|
|
|
A Comparison of Spinosin Content in Zizyphi Semen and Its Processed Products by Roasting
|
|
Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
Kim Byung-Soo
|
|
|
Abstract
|
|
|
|
The aim of this study was to compare the amount of spinosin in the 70% ethanol extracts of non-processed Zizyphi Semem (ZS) and processed ZS by roasting using a high-performance liquid chromatography equipped with photodiode array detector. Separation of the spinosin was used SunFireTM C18 analytical column (5 μm, 4.6×150 mm) using two mobile phase consisting of distilled water and acetonitrile, both with 1.0% (v/v) acetic acid. The flow rate was 1.0 mL/min and injection vol-ume was 10 μL. Calibration curve of the spinosin was y = 22339.45x+483.99 in tested concentration range (1.28-20.00 μg/mL) and correlation coefficient was 1.0000. In non-processed ZS sample, the amount of the spinosin was 0.94 m/g, while, the amount of the marker compound in processed ZS samples were 0.66-1.10 mg/g.
|
|
|
KEYWORD
|
|
|
Zizyphi Semem, Processed ZS, Roasting, Spinosin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|